More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 15.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human FGF9 standard: 2 vials
2. One 96-well plate precoated with anti-Human FGF9 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
FGF9 (fibroblast growth factor 9), also known as HBGF9 (heparin binding growth factor9) and GAF (glia activating factor), is a secreted glycoprotein of the FGF family. Human FGF9 shares 98% sequence identity with mouse and rat FGF9. FGF9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In nervous system, FGF9 functions as a glia activating factor to promote glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, and growth stimulation of glial tumors. It also exhibits activity in differentiation and survival of neuronal cells. In addition, FGF9 is required for male development. Once activated by SOX9, it is responsible for forming a feed-forward loop with Sox9, increasing the levels of both genes. The absence of FgF9 gene can cause an individual with X and Y chromosomes, to develop into a female. Essentially, FGF9 binds FGF R3 (IIIb) to transduce its signals in cells.