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Human anti-hepatitis A virus IgM antibody,anti-HAV ELISA Kit
Human anti-hepatitis A virus IgM antibody,anti-HAV ELISA Kit For research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit
Data sheet
| Validity | 12 months |
| Assay Range | Qualitative: Positive & Negative Control & Cut off |
| Sensitivity | 100% |
| Background | Infections normally contracted in early childhood, HAV can adversely affect the liver, but acute complications or chronic illness do not necessarily occur because Hepatitis A is a self-limited disease. Causes of the infection are connected to unsanitary conditions and poor hygiene within densely populated areas. Thus, the fecal-oral route of the disease is easily transmitted in this type of setting. One single infected person could cause an entire outbreak. Hepatitis A is a non-enveloped positive strand RNA virus with a linear single strand genome, encoded for only one known serotype. Within HAV are four major, structural polypeptides, concentrated exclusively in the cytoplasm of human hepatocites. An HAV infection causes a strong immunological response, and within a few days after the onset of symptoms, elevated levels of IgM, and subsequently IgG, are recognized. The identification of anti-HAV IgM serves as a critical serological marker in detection and observation of how the disease has manifested in a clinical setting. After approximately three weeks of exposure, increasing levels of anti-HAV IgM are detectable, with the highest titter occurring after four to six weeks. Concentration of IgM declines to nondetectable levels within six months after infection. |
| Assay principle | The principle method of the HAV-IgM ELISA is a solid phase, two-step incubation, antibody capture ELISA assay. In this HAV IgM ELISA kit, polystyrene microwell strips are pre-coated with antibodies to human immunoglobulin M proteins (anti-u chain). During the first incubation, the serum or plasma sample is added. At this point, any IgM antibodies will be captured in the wells. It is important to wash out sample components, specifically IgG antibodies. Then add HAV antigens conjugated to horseradish peroxidase (HRP-Conjugate), and any specific HAV IgM captured during the solid phase will be identified at this time. In the course of second incubation, the HRP-conjugated antigens will react only with the HAV IgM antibodies. Chromogen solutions are then added to the wells after washing to remove unbound-HRP-conjugate. The colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product when the (anti-u)-HAV-IgM)-(antigen-HRP) imunocomplex is present. After stopping the reaction with sulfuric acid, the blue color turns yellow. What can be measured at this point is the amount of color intensity proportional to the amount of antibody captured in the wells, and to the sample. Colorless wells appear if the HAV-IgM is negative. |
| INTENDED USE | The HAV IgM ELISA test is an enzyme-linked immunosorbent assay (ELISA) which is used for the qualitative determination of IgM-class antibodies to hepatitis A virus in human serum/plasma. The purpose of the HAV IgM ELISA Test is for diagnosis and management of patients infected with hepatitis A virus. |
| Sample | 100 ul serum/plasma |
| Specificity | 99% |