| Reconstitution |
1mg/ml in PBS (after reconstitution). |
| Clone |
PAT38E7A. |
| Applications |
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000. |
| Protein Background |
MRPS25, a 28S subunit protein, is a member of the ribosomal protein S5P family. An equivalent pseudogene is located on chromosome 4. |
| Synonyms |
Mitochondrial Ribosomal Protein S25, Mitochondrial 28S Ribosomal Protein S25, 28S Ribosomal Protein S25 Mitochondrial, MRP-S25, RPMS25, S25mt. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Type |
Mouse Anti Human Monoclonal. |
| Immunogen |
Anti-human MRPS25 mAb, clone PAT38E7A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MRPS25 protein 1-173 amino acids purified from E. coli. |
| Ig Subclass |
Mouse IgG2a heavy chain and Kappa light chain. |
| Purification Method |
MRPS25 antibody was purified by protein-A affinity chromatography. |
| Protein formulation |
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide. |
| Storage Procedures |
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Stability / Shelf Life |
12 months at -20°C. 1 month at 4°C. |
| Antibody Properties |
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Titer |
Antibody is shipped lyophilized at ambient temperature. |
| Protein content |
By direct ELISA, 1:10,000 dilution will yield 0.7 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
