More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| glutamate receptor, ionotropic, N-methyl D-aspartate 2B | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human NMDAR2B(1307-1320aa FVDLQKEEAALAPR), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GRIN2B |
Protein | Glutamate receptor ionotropic, NMDA 2B |
Uniprot ID | Q13224 |
Function | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity). |
Tissue Specificity | Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | AW490526 antibody|Glutamate[NMDA] receptor subunit epsilon 2 antibody|Glutamate[NMDA] receptor subunit epsilon-2 antibody|Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody|Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody|Glutamate receptor ionotropic NMDA2B antibody|Glutamate receptor subunit epsilon 2 antibody|Glutamate receptor, ionotropic, NMDA2B(epsilon 2) antibody|GRIN 2B antibody|GRIN2B antibody|hNR 3 antibody|hNR3 antibody|MGC142178 antibody|MGC142180 antibody|N methyl D asparate receptor channel subunit epsilon 2 antibody|N METHYL D ASPARTATE RECEPTOR CHANNEL SUBUNIT EPSILON 2 antibody|N methyl D aspartate receptor subtype 2B antibody|N methyl D aspartate receptor subunit 2B antibody|N methyl D aspartate receptor subunit 3 antibody|N-methyl D-aspartate receptor subtype 2B antibody|N-methyl-D-aspartate receptor subunit 3 antibody|NMDA NR2B antibody|NMDA R2B antibody|Nmdar2b antibody|NMDE2 antibody|NMDE2_HUMAN antibody|NME2 antibody|NR2B antibody|NR3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NMDAR2B antibody, ASA-B1389, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: U87 Cell Lysate
