More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| collagen, type III, alpha 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III(1200-1221aa VGAAAIAGIGGEKAGGFAPYYG), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | COL3A1 |
Protein | Collagen alpha-1(III) chain |
Uniprot ID | P02461 |
Function | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Belongs to the fibrillar collagen family. |
Aliases | CO3A1_HUMAN antibody|COL 3A1 antibody|COL3A1 antibody|Collagen alpha 1(III) chain antibody|Collagen alpha-1(III) chain antibody|Collagen III alpha 1 chain precursor antibody|Collagen III alpha 1 polypeptide antibody|Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody|Collagen type III alpha 1 antibody|Collagen type III alpha antibody|EDS4A antibody|Ehlers Danlos syndrome type IV, autosomal dominant antibody|Fetal collagen antibody|Type III collagen antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Collagen III antibody, ASA-B0472--1.jpg
All lanes: Anti Collagen III (ASA-B0472) at 0.5ug/ml
WB: Recombinant Human Collagen III Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti Collagen III (ASA-B0472) at 0.5ug/ml
WB: Recombinant Human Collagen III Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-Collagen III antibody, ASA-B0472--2.jpg
All lanes: Anti Collagen III(ASA-B0472) at 0.5ug/ml
Lane 1: MCF-4 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 139KD
Observed bind size: 139KD
All lanes: Anti Collagen III(ASA-B0472) at 0.5ug/ml
Lane 1: MCF-4 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 139KD
Observed bind size: 139KD
