More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD1a molecule | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human CD1a(158-173aa AKHFCKVLNQNQHEND). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD1A |
Protein | T-cell surface glycoprotein CD1a |
Uniprot ID | P06126 |
Function | Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. |
Tissue Specificity | Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Endosome membrane. Note: Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts. |
Sequence Similarities | |
Aliases | CD 1a antibody|CD1 antibody|CD1a antibody|CD1A antibody|CD1A Antigen antibody|CD1A antigen, a polypeptide antibody|CD1a molecule antibody|CD1A_HUMAN antibody|cluster of differentiation 1 A antibody|Cortical thymocyte antigen CD1A antibody|differentiation antigen CD1 alpha 3 antibody|Epidermal dendritic cell marker CD1a antibody|FCB 6 antibody|FCB6 antibody|HTA 1 antibody|HTA1 antibody|hTa1 thymocyte antigen antibody|OTTHUMP00000018907 antibody|R 4 antibody|R4 antibody|T 6 antibody|T Cell Surface Antigen Leu 6 antibody|T Cell Surface Antigen T6 antibody|T cell surface antigen T6/Leu 6 antibody|T Cell Surface Glycoprotein CD1A antibody|T-cell surface antigen T6/Leu-6 antibody|T-cell surface glycoprotein CD1a antibody|T6 antibody|Thymocyte Antigen CD1A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD1a antibody, ASA-B0361, Western blotting
All lanes: Anti CD1a (ASA-B0361) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti CD1a (ASA-B0361) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
