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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| toll-like receptor 4 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4(50-68aa DNLPFSTKNLDLSFNPLRH). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TLR4 |
Protein | Toll-like receptor 4 |
Uniprot ID | O00206 |
Function | Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species- specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. |
Tissue Specificity | Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Note: Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis. |
Sequence Similarities | Belongs to the Toll-like receptor family. |
Aliases | ARMD10 antibody|CD284 antibody|CD284 antigen antibody|Homolog of Drosophila toll antibody|hToll antibody|TLR 4 antibody|TLR4 antibody|TLR4_HUMAN antibody|Toll(Drosophila) homolog antibody|TOLL antibody|Toll Endotoxin Hyporesponsiveness antibody|Toll like receptor 4 antibody|Toll like receptor 4 precursor antibody|Toll-like receptor 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TLR4 antibody, ASA-B1874, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Anti-TLR4 antibody, ASA-B1874, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
