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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| transglutaminase 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human TGM2 (7-34aa LERCDLELETNGRDHHTADLCREKLVVR), different from the related mouse sequence by five amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TGM2 |
Protein | Protein-glutamine gamma-glutamyltransferase 2 |
Uniprot ID | P21980 |
Function | Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the transglutaminase superfamily. Transglutaminase family. |
Aliases | ALPHA SUBUNIT antibody|C polypeptide antibody|EC 2.3.2.13 antibody|G alpha h antibody|G protein alpha subunit Gh class antibody|G[a]h antibody|Gh CLASS G ALPHA h antibody|GNAH antibody|GNAH G PROTEIN antibody| Guanine nucleotide binding protein H polypeptide antibody| H POLYPEPTIDE antibody|Protein glutamine gamma glutamyltransferase 2 antibody| Protein glutamine gamma glutamyltransferase antibody|Protein-glutamine gamma-glutamyltransferase 2 antibody|TG 2 antibody|TG(C) antibody|TG2 antibody| TGase C antibody| TGase H antibody| TGase-2 antibody|TgaseII antibody|TGC antibody|TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody|TGM 2 antibody|TGM2 antibody|TGM2_HUMAN antibody|Tissue transglutaminase antibody| Tissue type transglutaminase antibody|Transglutaminase 2 antibody|Transglutaminase 2 C polypeptide antibody|Transglutaminase C antibody|Transglutaminase H antibody| Transglutaminase-2 antibody|tTG antibody|tTGas antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TGM2 antibody, ASA-B1854, Western blotting
All lanes: Anti TGM2 (ASA-B1854) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 77KD
Observed bind size: 77KD
All lanes: Anti TGM2 (ASA-B1854) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 77KD
Observed bind size: 77KD
Anti- TGM2 antibody, ASA-B1854, IHC(P)
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue
Anti- TGM2 antibody, ASA-B1854, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue