More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A4 (57-85aa DDTRHSIPATTTTLVAELHQGERETWGKK), different from the related mouse and rat sequences by six amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC6A4 |
Protein | Sodium-dependent serotonin transporter |
Uniprot ID | P31645 |
Function | Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. |
Tissue Specificity | Expressed in platelets (at protein level). |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Note: Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. |
Sequence Similarities | Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily. |
Aliases | 5 HTT antibody|5 HTTLPR antibody|5 hydroxytryptamine (serotonin) transporter antibody|5 hydroxytryptamine transporter antibody|5HT transporter antibody|5HTT antibody|hSERT antibody|HTT antibody|Na+/Cl- dependent serotonin transporter antibody|OCD1 antibody| SC6A4_HUMAN antibody|Serotonin transporter 1 antibody|SERT antibody|SERT1 antibody|SLC6A4 antibody|Sodium dependent serotonin transporter antibody|Sodium-dependent serotonin transporter antibody| Solute carrier family 6 (neurotransmitter transporter) member 4 antibody|solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 antibody| Solute carrier family 6 member 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SLC6A4 antibody, ASA-B1718, Western blotting
All lanes: Anti SLC6A4 (ASA-B1718) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 70KD
Observed bind size: 95KD
All lanes: Anti SLC6A4 (ASA-B1718) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 70KD
Observed bind size: 95KD
Anti- SLC6A4 antibody, ASA-B1718, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti- SLC6A4 antibody, ASA-B1718, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue