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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| RAD51 recombinase | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Rad51 recombinant protein (Position: M1-E258). Human Rad51 shares 98% amino acid (aa) sequence identity with mouse Rad51. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RAD51 |
Protein | DNA repair protein RAD51 homolog 1 |
Uniprot ID | Q06609 |
Function | Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. |
Tissue Specificity | Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. |
Sub-cellular localization | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note: Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. |
Sequence Similarities | Belongs to the RecA family. RAD51 subfamily. |
Aliases | BRCA1/BRCA2 containing complex, subunit 5 antibody|BRCC 5 antibody|BRCC5 antibody|DNA repair protein RAD51 homolog 1 antibody|DNA repair protein rhp51 antibody|E coli RecA homolog antibody|Homolog of E coli RecA antibody|homolog of S cerevisiae RAD51 antibody|HRAD51 antibody|HsRad51 antibody|HsT16930 antibody|MRMV2 antibody|Rad 51 antibody|RAD51 antibody|RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody|RAD51 homolog A antibody|RAD51 homolog antibody|RAD51 homolog S. cerevisiae antibody|RAD51 recombinase antibody|RAD51 S cerevisiae homolog antibody|RAD51, S. cerevisiae, homolog of antibody|RAD51_HUMAN antibody|RAD51A antibody|RECA antibody|RecA homolog E. coli antibody|RecA like protein antibody|RecA, E. coli, homolog of antibody|recombination protein A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Rad51 antibody, ASA-B1606, Western blotting
All lanes: Anti Rad51 (ASA-B1606) at 0.5ug/ml
Lane 1: 22RV1 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Lane 6: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti Rad51 (ASA-B1606) at 0.5ug/ml
Lane 1: 22RV1 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Lane 6: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
Anti- Rad51 antibody, ASA-B1606, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
Anti- Rad51 antibody, ASA-B1606, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue