More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| myosin, heavy chain 11, smooth muscle | Monoclonal | IgG | Mouse | Chicken, Human, Rabbit | IHC-P, IHC-F, WB | Ascites |
Immunogen |
| Human uterus smooth muscle extract. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MYH11 |
Protein | Myosin-11 |
Uniprot ID | Human(P35749), Mouse(O08638), Rat(Q63862) |
Function | Muscle contraction. |
Tissue Specificity | Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles. |
Sub-cellular localization | Melanosome. |
Sequence Similarities | Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. |
Aliases | AAT4|FAA4|SMHC|SMMHC|KIAA0866|Myosin heavy chain 11|Myosin heavy chain, smooth muscle isoform |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.25-0.5μg/ml | Human, chicken, rabbit | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, chicken, rabbit | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, chicken, rabbit | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
