More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| LIM and SH3 protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human LASP1(16-30aa EKVNCLDKFWHKACF), identical to the related rat sequence and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LASP1 |
Protein | LIM and SH3 domain protein 1(LASP-1) |
Uniprot ID | Q14847 |
Function | Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Note: Associated with the F- actin rich cortical cytoskeleton. |
Sequence Similarities | Contains 1 LIM zinc-binding domain. |
Aliases | LASP 1 antibody|LASP-1 antibody|LASP1 antibody|LASP1_HUMAN antibody|LIM and SH3 domain protein 1 antibody|LIM and SH3 protein 1 antibody|Metastatic lymph node gene 50 protein antibody|MLN 50 antibody|MLN50 antibody|OTTHUMP00000164238 antibody|OTTHUMP00000164239 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-LASP1 antibody, ASA-B1158, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Intestine Tissue Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: A431 Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: 293T Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Intestine Tissue Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: A431 Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: 293T Cell Lysate
Anti-LASP1 antibody, ASA-B1158, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-LASP1 antibody, ASA-B1158, ICC
ICC: A549 Cell
ICC: A549 Cell