More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 60kDa protein 1(chaperonin) | Monoclonal | IgG | Mouse | Chicken, Human, Rat | IHC-P, WB | Ascites |
Immunogen |
| Recombinant human heat shock protein 60(HSP60) |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPD1 |
Protein | 60 kDa heat shock protein, mitochondrial |
Uniprot ID | Human(P10809), Mouse(P63038), Rat(P63039) |
Function | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion matrix. |
Sequence Similarities | Belongs to the chaperonin (HSP60) family. |
Aliases | SPG13|GROEL|CPN60|HuCHA60|spastic paraplegia 13(autosomal dominant)|CHAPERONIN, 60-KD|Mitochondrial matrix protein P1|P60 lymphocyte protein|Heat shock protein 60|60 kDa chaperonin|Chaperonin 60 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 2-4μg/ml | Human, chicken, rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 4-8μg/ml | Human, chicken, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
