More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 60kDa protein 1(chaperonin) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60(503-516aa YDAMLGDFVNMVEK), different from the related human sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPD1 |
Protein | 60 kDa heat shock protein, mitochondrial |
Uniprot ID | P63039 |
Function | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion matrix . |
Sequence Similarities | |
Aliases | 60 kDa chaperonin antibody|60 kDa heat shock protein mitochondrial antibody|60 kDa heat shock protein, mitochondrial antibody|CH60_HUMAN antibody|Chaperonin 60 antibody|Chaperonin antibody|Chaperonin, 60-KD antibody|CPN 60 antibody|CPN60 antibody|fa04a05 antibody|GROEL antibody|GroEL Homolog antibody|Heat shock 60kD protein 1 chaperonin antibody|Heat shock 60kDa protein 1 antibody|Heat shock protein 1(chaperonin) antibody|Heat Shock Protein 60 antibody|Heat shock protein 65 antibody|HLD4 antibody|Hsp 60 antibody|Hsp-60 antibody|HSP 65 antibody|HSP-60 antibody|HSP60 antibody|HSP65 antibody|HSPD 1 antibody|HSPD1 antibody|HuCHA60 antibody|Mitochondrial matrix protein P1 antibody|P60 lymphocyte protein antibody|Short heat shock protein 60 Hsp60s1 antibody|Spastic paraplegia 13 antibody|SPG 13 antibody|SPG13 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSP60 antibody, ASA-B0916, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: CEM Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: COLO320 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: CEM Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: COLO320 Cell Lysate
Anti-HSP60 antibody, ASA-B0916, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
Anti-HSP60 antibody, ASA-B0916, ICC
ICC: SMMC Cell
ICC: SMMC Cell