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CD163 Antibody

ASA-B0355

$330.00

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Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
CD163 molecule Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

E. coli-derived human CD163 recombinant protein(Position: F1056-L1165).

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CD163

Protein

 Scavenger receptor cysteine-rich type 1 protein M130

Uniprot ID

 Q86VB7

Function

 Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

Tissue Specificity

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

Sub-cellular localization

 Soluble CD163: Secreted.

Sequence Similarities

 Contains 9 SRCR domains.

Aliases

 C163A_HUMAN antibody|CD 163 antibody|CD163 antibody|CD163 antigen antibody|CD163 molecule antibody|Hemoglobin Scavenger Receptor antibody|M130 antibody|M130 antigen precursor antibody|Macrophage associated antigen antibody|MM130 antibody|OTTHUMP00000238617 antibody|OTTHUMP00000238618 antibody|OTTHUMP00000238619 antibody|OTTHUMP00000238620 antibody|Scavenger receptor cysteine rich type 1 protein M130 antibody|sCD163 antibody|Soluble CD163 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD163 antibody, ASA-B0355, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-CD163 antibody, ASA-B0355, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-CD163 antibody, ASA-B0355, Western blotting
All lanes: Anti CD163 (ASA-B0355) at 0.5ug/ml
Lane 1: Recombinant Human CD163 Protein 10ng
Lane 2: Recombinant Human CD163 Protein 5ng
Lane 3: Recombinant Human CD163 Protein 2.5ng
Predicted bind size: 35KD
Observed bind size: 35KD

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