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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| arylsulfatase A | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA (454-482aa QALKQLQLLKAQLDAAVTFGPSQVARGED), different from the related mouse sequence by six amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ARSA |
Protein | Arylsulfatase A |
Uniprot ID | P15289 |
Function | Hydrolyzes cerebroside sulfate. |
Tissue Specificity | |
Sub-cellular localization | Lysosome. |
Sequence Similarities | Belongs to the sulfatase family. |
Aliases | arsA antibody|ARSA_HUMAN antibody|arylsulfatase A antibody|Arylsulfatase A component C antibody|As 2 antibody|AS A antibody|As2 antibody|ASA antibody| AW212749 antibody| C230037L18Rik antibody|Cerebroside-sulfatase antibody| metachromatic leucodystrophy antibody|MGC125207 antibody|MLD antibody| OTTHUMP00000196546 antibody|OTTHUMP00000196548 antibody|TISP73 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ARSA antibody, ASA-B0154, Western blotting
All lanes: Anti ARSA (ASA-B0154) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Pancreas Tissue Lysate at 50ug
Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Kidney Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted band size: 54KD
Observed band size: 54KD
All lanes: Anti ARSA (ASA-B0154) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Pancreas Tissue Lysate at 50ug
Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Kidney Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted band size: 54KD
Observed band size: 54KD
Anti-ARSA antibody, ASA-B0154,IHC(P)
Rat Lung Tissue
Rat Lung Tissue
Anti-ARSA antibody, ASA-B0154,IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue