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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| apoptosis inhibitor 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5 (487-504aa KYSSNLGNFNYERSLQGK), different from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | API5 |
Protein | Apoptosis inhibitor 5(API-5) |
Uniprot ID | Q9BZZ5 |
Function | Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. |
Tissue Specificity | Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the API5 family. |
Aliases | AAC 11 antibody|AAC-11 antibody|AAC11 antibody|Antiapoptosis clone 11 protein antibody|API 5 antibody|API-5 antibody|API5 antibody|API5 like 1 antibody|API5_HUMAN antibody|API5L1 antibody|Apoptosis inhibitor 5 antibody|Cell migration inducing gene 8 protein antibody|Cell migration-inducing gene 8 protein antibody|Fibroblast growth factor 2 interacting factor 2 antibody|Fibroblast growth factor 2-interacting factor antibody|FIF antibody|MIG 8 antibody|MIG8 antibody|Migration inducing protein MIG8 antibody|Protein XAGL antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Apoptosis inhibitor 5 antibody, ASA-B0126, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Placenta Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: CEM Cell Lysate
Lane 8: SMMC Cell Lysate
Lane 9: COLO320 Cell Lysate
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Placenta Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: CEM Cell Lysate
Lane 8: SMMC Cell Lysate
Lane 9: COLO320 Cell Lysate
Anti-Apoptosis inhibitor 5 antibody, ASA-B0126, IHC(P)
Rat Brain Tissue
Rat Brain Tissue
Anti-Apoptosis inhibitor 5 antibody, ASA-B0126, ICC
HELA Cells
HELA Cells