More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Janus kinase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human JAK1(970-984aa KNKNKINLKQQLKYA), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | JAK1 |
Protein | Tyrosine-protein kinase JAK1 |
Uniprot ID | P23458 |
Function | Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. |
Tissue Specificity | Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. |
Sub-cellular localization | Endomembrane system; Peripheral membrane protein. Note: Wholly intracellular, possibly membrane associated. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. |
Aliases | EC 2.7.10.2 antibody|JAK 1 antibody|JAK 1A antibody|JAK 1B antibody|JAK-1 antibody|JAK1 antibody|JAK1_HUMAN antibody|JAK1A antibody|JAK1B antibody|Janus kinase 1(a protein tyrosine kinase) antibody|Janus Kinase 1 antibody|JTK3 antibody|Tyrosine protein kinase JAK 1 antibody|Tyrosine protein kinase JAK1 antibody|Tyrosine-protein kinase JAK1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- JAK1 antibody, ASA-B1084, Western blotting
All lanes: Anti JAK1 (ASA-B1084) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 133KD
Observed bind size: 133KD
All lanes: Anti JAK1 (ASA-B1084) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 133KD
Observed bind size: 133KD
