More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 2 | Polyclonal | IgG | Rabbit | Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived rat IL2 recombinant protein (Position: A21-Q155). Rat IL2 shares 66% and 80% amino acid (aa) sequences identity with human and mouse IL2, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL2 |
Protein | Interleukin-2 |
Uniprot ID | P17108 |
Function | Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | Aldesleukin antibody|IL 2 antibody|IL-2 antibody|IL2 antibody|IL2_HUMAN antibody|Interleukin 2 antibody|Interleukin-2 antibody|Interleukin2 antibody|Involved in regulation of T cell clonal expansion antibody|Lymphokine antibody|OTTHUMP00000164090 antibody|POIL2 antibody|T-cell growth factor antibody|TCGF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL2 antibody, ASA-B1013--1.jpg
All lanes: Anti-IL2(ASA-B1013) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 40ug
Lane 2: Rat Liver Tissue Lysate at 40ug
Lane 3: NRK Whole Cell Lysate at 40ug
Lane 4: PC12 Whole Cell Lysate at 40ug
Lane 5: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 18KD
Observed bind size: 18KD
All lanes: Anti-IL2(ASA-B1013) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 40ug
Lane 2: Rat Liver Tissue Lysate at 40ug
Lane 3: NRK Whole Cell Lysate at 40ug
Lane 4: PC12 Whole Cell Lysate at 40ug
Lane 5: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 18KD
Observed bind size: 18KD
