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Cynomolgus CD32a/Fc gamma RIIA/FCGR2A ELISA Kit

Catalog #: NS-E10235

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Cynomolgus CD32a/Fc gamma RIIA/FCGR2A ELISA Kit

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Data sheet

Assay Range	7.8 - 500 pg/ml

More info

Sensitivity

5 pg/mL

Size

96T

Storage

Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC

Assay Principle

Sandwich ELISA

Sample volume

100 µL final volume, dilution factor varies on samples.

Detection Method

Chromogenic

Kit Components

1. Recombinant cynomolgus CD32a standard: 1 ng/vial - 2.

2. One 96-well plate precoated with anti-cynomolgus CD32a Ab

3. Sample diluent buffer: 12 mL x 1

4. HRP conjugated anti-cynomolgus CD32a Ab: 1 vial, dilution 1:500.

5. Detection Antibody diluent buffer: 12 mL x 1

6. 20 x Wash Solution: 25 ml x 1.

7. TMB color developing agent A: 6 ml.

8. TMB color developing agent B: 6 ml.

9. TMB stop solution: 6ml.

Background

Low affinity immunoglobulin gamma Fc region receptor II-a, also known as IgG Fc receptor II-a, Fc-gamma-RIIa, CD32a and FCGR2A, is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16.CD32 is a low affinity receptor for IgG. The activating isoform, CD32a, is expressed on monocytes, neutrophils, platelets and dendritic cells. Ligation of this receptor leads to cell activation. The inhibitory CD32b is expressed on B cells and myeloid dendritic cells. Ligation of CD32b on B cells down regulates antibody production and may promote apoptosis.

standard curve

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protocol

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