More info
Principle
Indirect ELISA: Antigen Coated Plate
Detection Range
Qualitative Positive; Negative control & Cut off
Size
96T
Sensitivity
99%
Specificity
97%
Assay time
~ 75 min
Shelf Life
12 ~ 18 Months from the Manufacturing Date
Storage
Store at 2 - 8ºC.
Sample volume
10 µL Serum or Plasma
Detection Method
Chromogenic
Kit Components
1. Assay calibrator: 1vial
2. One 96-well plate precoated with Toxoplasma Gondii
3. Assa diluent buffer: 12 mL x 1
4. HRP conjugated: 1 vial,
5. Positive Control: 1 vial
6. Negative Control:1vial
7. 20 x Wash buffer: 1 vial
8. TMB substrat: 12 ml.
9. TMB stop solution: 12 ml.
Background
A single-celled parasite called Toxoplasma gondii causes a disease known as toxoplasmosis. Although mild, flu-like symptoms occasionally occur during the first few weeks following exposure, infection with T. gondii generally produces no symptoms in healthy human adults. Severe toxoplasmosis, causing damage to the brain, eyes, or other organs, can develop from an acute Toxoplasma infection or one that had occurred earlier in life and is now reactivated. Toxoplasma gondii IgG antibodies usually appear within 1 to 2 weeks of the infection, peak within 1 to 2 months, fall at variable rates, and usually persist for life. The titer does not correlate with the severity of illness.