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Carbonyl Reductase-1, Monoclonal Mouse Anti Human Antibody ( CBR1 Antibody )
TypeMouse Anti Human Monoclonal.Clone4E12.ImmunogenAnti-human CBR1 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CBR1 protein.Ig SubclassMouse IgG2a heavy chain
Data sheet
| Clone | 4E12. |
| Applications | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:1,000. |
| Protein Background | CBR1 is a NADPH-dependent, monomeric, and cytosolic enzyme which is related to a family of short-chain dehydrogenases/reductases. CBR1 protein (277 a.a.) is broadly spread in human tissues such as liver, epidermis, stomach, small intestine, kidney, neuronal cells, and smooth muscle fiber. CBR1 metabolizes numerous toxic environmental quinones and pharmacological relevant substrates such as the anticancer drug, doxorubicin. The best substrates of CBR1 are quinones, including ubiquinone-1 and tocophrolquinone (vitamin E). |
| Synonyms | Carbonyl Reductase 1, SDR21C1, Prostaglandin-E(2) 9-reductase, NADPH-dependent carbonyl reductase 1, CBR, hCBR1, 15-hydroxyprostaglandin dehydrogenase, CRN. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Type | Mouse Anti Human Monoclonal. |
| Immunogen | Anti-human CBR1 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CBR1 protein. |
| Ig Subclass | Mouse IgG2a heavy chain and Kappa light chain |
| Purification Method | Recombinant human CBR1 (1-277 aa ) purified from E. coli |
| Protein formulation | 1mg/ml containing Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
| Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. |