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FLAG Peptide, Mouse Antibody Peroxidase Conjugated ( FLAG-HRP Antibody )
TypeMouse Antibody Monoclonal.ClonePFLAGHRPSHG.DescriptionMonoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.Ig SubclassMouse IgG2b.Formulation1×PBS & 50% glycerol.Stability / Shelf Life2 years
Data sheet
| Clone | PFLAGHRPSHG. |
| Formulation | 1PBS & 50% glycerol. |
| Applications | Western Blot. |
| Description | Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH. |
| Protein Background | A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide.The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins. |
| Type | Mouse Antibody Monoclonal. |
| Ig Subclass | Mouse IgG2b. |
| link | Purified by proprietary chromatographic technique. |