Data sheet
| Background | Interleukin 6 (IL-6), also known as B-cell stimulatory factor 2(BSF-2), CTL differentiation factor (CDF), Hybridoma growth factor, or Interferon beta-2 (IFN-beta-2), is a glycosylated cytokine belonging to the IL-6 family. Mature human IL-6 is a 183 amino acid (aa) polypeptide and shares 39% aa sequence identity with mouse and rat IL-6. IL-6 is expressed in many different cells such as CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells, sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells as well as pancreatic islet beta cells. IL-6 binds to a cell surface heterodimeric receptor complex composed of IL-6 R and gp130 which is also a common component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. IL-6 is required for fever and the acute phase response in the liver, and it plays an important role in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. |
| Alternate Names | B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric ELISA |
| Assay Time | 4.5 hours |
| Sample Type | Plasma, Serum, cell culture medium |
| Assay Range | 125-8000 pg/ml |
| Sensitivity | 10 pg/ml |
| Specificity | No cross-reaction with other related substances detected |
| Recovery | 92 - 106% |
| Precision | Intra-Assay 4.3%; Inter-Assay 8.7%. |
| Sample Volume | 100 ul |
| Dilution Factors | Varies. Need to test before a large-scale assay. |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Gene ID | 399500 |
| UniProt ID | P26893 |
More info
Kit Components
1. Recombinant Porcine IL-6 standard: 2 vials
2. One 96-well plate coated with Porcine IL-6 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5.Streptavidin-HRP: 1 vial
6. Antibody diluent buffer:12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9. Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Citations:
1. Sec62 Suppresses Foot-and-Mouth Disease Virus Proliferation by Promotion of IRE1α–RIG-I Antiviral Signaling, Han S et al, J Immunol June 5, 2019, ji1801546; DOI: https://doi.org/10.4049/jimmunol.1801546
2. Foot-and-mouth disease virus infection suppresses autophagy and NF-кB antiviral responses via degradation of ATG5-ATG12 by 3Cpr, Fan X, et al, Cell Death & Disease volume 8, page e2561 (2017)