Data sheet
| Specificity | reactive with Mouse L-Selectin，reactivity with other related proteins not observed |
| Assay Range | 125-8000 pg/ml |
| Sensitivity | 12 pg/ml |
| Size | 96 Tests |
| Storage | 2-8℃ |
| Background | L-selectin, also known as CD62L, is a cell adhesion molecule found on lymphocytes and the preimplantation embryo. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL is a cell surface component that is a member of a family of adhesion/homing receptors that play important roles in lymphocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4-binding proteins. |
| Assay principle | This Mouse L-Selectin ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse L-Selectin specific antibodies were precoated onto 96-well plates. The Mouse L-Selectin specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse L-Selectin amount of sample captured in plate. |
| Sample type | serum，plasma，cell culture supernatant |
| Assay duration | 4 hours |
| Assay format | Sandwich |
| Alternative Name of Analyte | SELL；CD62L；LAM1；LECAM1；LEU8；LNHR；LSEL；LYAM1；PLNHR；TQ1 |
More info
L-selectin, also known as CD62L, is a cell adhesion molecule found on lymphocytes and the preimplantation embryo. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. High expression of L-selectin on bone marrow progenitor cells is an early sign of cells becoming committed to lymphoid differentiation.