| Specificity |
reactive with Mouse LDL R，reactivity with other related proteins not observed |
| Assay Range |
62.5-4000 pg/ml |
| Sensitivity |
6 pg/ml |
| Size |
96 Tests |
| Storage |
2-8℃ |
| Background |
The Low-Density Lipoprotein (LDL) Receptor is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). In mouse, the LDL receptor protein is encoded by the LDLR gene. It belongs to the Low density lipoprotein receptor gene family.Brown and Goldstein won a Nobel Prize for their identification of the Low Density Lipoprotein (LDL) receptor in 1985 while they were studying familial hypercholesterolemia. |
| Assay principle |
This Mouse LDL R ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse LDL R specific antibodies were precoated onto 96-well plates. The Mouse LDL R specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse LDL R amount of sample captured in plate. |
| Sample type |
serum，plasma，cell culture supernatant |
| Assay duration |
4 hours |
| Assay format |
Sandwich |
| Alternative Name of Analyte |
LDLR；FH；FHC；LDLCQ2 |
