More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse TNFRSF18 standard: 2 vials
2. One 96-well plate precoated with anti- Mouse TNFRSF18 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR, (activation-inducible TNF R family member), Tumor necrosis factor receptor superfamily member 18 (TNFRF18), or CD357, is a type I transmembrane protein belonging to the TNF R family. Human GITR shares 55% homology with mouse GITR. Expression of GITR has been found in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes, and this expression is upregulated by antigen stimulation or by treatment with antiCD3 plus antiCD28, or PMA plus ionomycin. In mice, multiple alternative spliced isoforms of GITR gene have been observed. The first reported isoform is a 228 amino acid (aa) precursor that composed of a 19 aa signal sequence, a 134 aa extracellular region with four potential N-linked glycosylation sites and three cysteine-rich pseudo-repeats, a 21 aa transmembrane segment, and a 54 aa cytoplasmic domain containing a PxQ/EE motif that is known to associate with TRAF2 which is a characteristic of TNFRSF members with costimulatory functions. Human GITR ligand (TNFSF18) is a 177 aa polypeptide belonging to the TNF superfamily. It is shown that Ligation of GITR can activate NFκB through TRAF2, and protect T cells from TCR activation-induced cell death. Based on the data available, it is proposed that GITR might play a key role in dominant immunological self-tolerance maintained by CD25+/CD4+ regulatory T cells.