More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 5.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse Galectin-1 standard: 2 vials.
2. One 96-well plate precoated with anti- Mouse Galectin-1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Galectin-1, also known as LGALS1 (lectin, galactoside-binding, soluble 1), is a member of carbohydrate binding protein family. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments, or secreted via non-classical pathways. Galectins are produced in various cell types including endothelial cells, connective tissue fibroblasts, thymic stromal cells, tumor cells, muscle cells, platelets, regulatory T cells, and activated tissue macrophages, B cells, T cells and dendritic cells. Mouse Galectin-1shares 88% and 96% aa sequence identity with human and rat counterparts, respectively. Galectin-1 preferentially binds laminin, fibronectin, 90K/Mac2BP, CD45, CD43, CD7, CD2, CD3, integrins α4β1, α5β1 and α4β7, and ganglioside GM1. Galectin-1 has exhibited multiple functions in negative selection of developing T cells, immunosuppression by regulatory T cells, resolution of the inflammatory response, and inhibition of immune cell migration, inflammatory cytokine production, and mast cell degranulation. It selectively controls T cell survival by inducing apoptosis of activated Th1 and Th17 cells, which express Galectin-1binding glycans, while promoting Th2 cell survival where glycans are sialylated and less recognized. It also induces apoptosis of immature thymocytes. Galectin-1 secreted from bone marrow stromal cells aids B lymphocyte development by contributing to pre-B cell integrin adhesion and receptor signaling. Its immunosuppressive properties may allow tumor cells to evade immune detection. Galectin-1 also is highly expressed at the maternal-fetal interface and contributes to fetal immune privilege. In addition to the primary roles in immunoregulation, Galectin-1 can modulate cell-cell and cell-matrix interactions, and can promote either cell attachment or detachment depending on the cell type and developmental stage.Hashimoto's thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy.