More info
Product name
Mouse MMP-9 ELISA Kit
Catalog No.
FM-E100103
Size
96T
Range
156pg/mL-10,000pg/mL
Sensitivity
Less Than 20pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
serum, plasma, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse MMP-9 standard: 10ng/tube-2.
2. One 96-well plate precoated with anti- mouse MMP-9 antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- mouse MMP-9 antibody : 130µL, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.5 The standard product used in this kit is recombinant mouse MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
Mouse MMP-9 ELISA Kit-1X96 Well Plate Image