Data sheet
| Specificity | No detectable cross-reactivity with any other cytokine. |
More info
Product name
Mouse L-Selectin ELISA Kit
Catalog No.
FM-E100124
Size
96T
Range
62.5pg/mL-4000pg/mL
Sensitivity
Less Than 5 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
For quantitative detection of mouse L-Selectin in serum,
plasma, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse L-Selectin standard: 10ng/tube-2.
2. One 96-well plate precoated with anti- mouse L-Selectin antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- mouse L-Selectin antibody: 130µL, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL L-selectinis a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point .
Mouse L-Selectin ELISA Kit-1X96 Well Plate Image