More info
Product name
Mouse IFNγ ELISA Kit
Catalog No.
FM-E100040
Size
96T
Range
31.2pg/mL-2000pg/mL
Sensitivity
Less Than 5 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
serum, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse IFNγ standard: 10ng/tube-2.
2. One 96-well plate precoated with anti- mouse IFNγ antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- mouse IFNγ antibody: 130µL, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.5 The standard product used in this kit is recombinant mouse IFNγ, consisting of 134 amino acid with the molecular mass of 15.6KDa.