More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse Cathepsin D standard: 2 vials.
2. One 96-well plate precoated with anti- Mouse Cathepsin D Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
The cathepsins are a group of lysosomal proteases that are active in acidic environments and play an important role in protein degradation. Currently, 11 human cathepsins (B, C, F, H, K, L, O, S, V, W and X) have been shown to have broad substrate specificity. For example, Cathepsins A and G are serine proteases and cathepsins D and E are aspartic proteases. Cathepsins are synthesized as inactive proenzymes and processed to become mature and active enzymes. Mouse cathepsin D is synthesized as a precursor protein, consisting of a signal peptide, a propeptide, and a mature chain which can further be processed to the light and heavy chains. Cathepsin D is expressed in many different cells and overexpressed in breast cancer cells. It plays a major role in lysosomal protein degradation and also involved in the presentation of antigenic peptides. Deficiency of Cathepsin D in mice showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is required for regulating cell growth and tissue homeostasis. It is reported that Cathepsin D in human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis.