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Data sheet
| Assay Range | 12.5 - 800 pg/ml |
| Sensitivity | 10 pg/ml |
| Size | 96T |
| Storage | Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C. |
| Expiration | Six months at 2 - 8 ⁰C. |
| Application | For quantitative determination of hemagglutinin. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Sample type | Serum, plasma, body fluids. |
More info
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant H1N1hemagglutinin standard: 4ng/vial - 2.
2. One 96-well plate precoated with anti-H1N1 hemagglutinin antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-H1N1 hemagglutinin antibody: 50µL, dilution 1:250.
5. Detection Antibody diluention buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Swine influenza, also called pig influenza, swine flu, hog flu and pig flu, is an infection caused by any one of several types of swine influenza viruses. Swine influenza virus (SIV) or swine-origin influenza virus (S-OIV) is any strain of the influenza family of viruses that is endemic in pigs. Transmission of the virus from pigs to humans is not common and does not always lead to human flu, often resulting only in the production of antibodies in the blood. Hemagglutinin (HA), which binds to sialic acid (SA)-containing receptors on host cells, is the protein that produces neutralizing antibodies. Hemagglutinin plays a major role in the determination of host range restriction and virulence.
