Human Cystatin C ELISA Kit

Kit Components

 1. Recombinant Human Cystatin C standard: 2 vials

 2. One 96-well plate coated with Human Cystatin C  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1

10. Washing solution (20x): 25 mL x1

Background

Cystatin C, also known as neuroendocrine basic polypeptide, or post-gamma-globulin, is an extracellular cysteine protease inhibitor belonging to the cystatin superfamily. The cysteine proteases of the papain family are major targets of Cystatin C. Cystatin C forms reversible 1:1 complexes with its target enzymes in competition with their substrates. Cystatin C is produced ubiquitously and present in all biological fluids. It has been implicated in the pathogenesis of many diseases, such as inflammation and tumor metastasis. A single nucleotide mutation generating a replacement of Leucine 68 to glutamine in Cystatin C leads to hereditary Cystatin C amyloid angiopathy. Since Cystatin C also binds amyloid β and reduces its aggregation and deposition, it appears to be a potential target in the treatment of Alzheimer's disease. On the other hand, Cystatin C levels have been reported to be higher in subjects with Alzheimer's disease. Cystatin C has also been used as an important biomarker for renal function assessment. Because of its small size and basic pI, Cystatin C is freely filtered by the glomerulus. It is then reabsorbed by tubular epithelial cells and subsequently metabolized so that it does not return to the bloodstream. Therefore, Cystatin C serum concentration correlates closely to the glomerular clearance rate.