More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cadherin 5, type 2(vascular endothelium) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human VE Cadherin(766-784aa RFKMLAELYGSDPREELLY), different from the related mouse and rat sequences by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDH5 |
Protein | Cadherin-5 |
Uniprot ID | P33151 |
Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. |
Tissue Specificity | Endothelial tissues and brain. |
Sub-cellular localization | Cell junction. |
Sequence Similarities | |
Aliases | 7B 4 antibody|7B4 antibody|7B4 antigen antibody|CADH5_HUMAN antibody|Cadherin 5 antibody|Cadherin 5 type 2 antibody|Cadherin 5, type 2(vascular endothelium) antibody|Cadherin 5, type 2, VE cadherin(vascular epithelium) antibody|cadherin, vascular endothelial antibody|cadherin, vascular endothelial, 1 antibody|Cadherin-5 antibody|Cadherin5 antibody|CD 144 antibody|CD144 antibody|CD144 antigen antibody|CDH 5 antibody|CDH5 antibody|CDH5 protein antibody|Endothelial specific cadherin antibody|FLJ17376 antibody|OTTHUMP00000174777 antibody|Vascular endothelial cadherin antibody|Vascular epithelium cadherin antibody|VE Cad antibody|VE-cadherin antibody|VEC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-VE Cadherin antibody, ASA-B1961, Western blotting
All lanes: Anti VE Cadherin (ASA-B1961) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 130KD
All lanes: Anti VE Cadherin (ASA-B1961) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 130KD
Anti-VE Cadherin antibody, ASA-B1961, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-VE Cadherin antibody, ASA-B1961, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue