TRPM2 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
transient receptor potential cation channel, subfamily M, member 2	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TRPM2(91-106aa CGYTHEQHLEEATKPH), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	TRPM2
Protein	Transient receptor potential cation channel subfamily M member 2
Uniprot ID	O94759
Function	Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.
Tissue Specificity	Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.
Sub-cellular localization	Membrane.
Sequence Similarities	Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.
Aliases	EREG 1 antibody|EREG1 antibody|Estrogen responsive element associated gene 1 antibody|Estrogen responsive element associated gene 1 protein antibody|Estrogen-responsive element-associated gene 1 protein antibody|KNP 3 antibody|KNP3 antibody|Long transient receptor potential channel 2 antibody|LTRPC 2 antibody|LTrpC-2 antibody|LTRPC2 antibody|MGC133383 antibody|NUDT9H antibody|NUDT9L1 antibody|putative Ca2+ channel protein antibody|transient receptor potential cation channel subfamily M member 2 antibody|Transient receptor potential channel 7 antibody|TRPC 7 antibody|TRPC7 antibody|TRPM 2 antibody|Trpm2 antibody|TRPM2_HUMAN antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information