Synaptopodin Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
synaptopodin	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Synaptopodin(495-509aa TSQMERSPMLERRHF), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SYNPO
Protein	Synaptopodin(SYNPO)
Uniprot ID	Q8N3V7
Function	Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity (By similarity).
Tissue Specificity	Expressed in cerebral cortex.
Sub-cellular localization	Cytoplasm, cytoskeleton . Cell junction, tight junction . Perikaryon . Cell projection, dendritic spine . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse . Note: Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Associated with dendritic spines of a subset of synapses (By similarity).
Sequence Similarities	Belongs to the synaptopodin family.
Aliases	KIAA1029 antibody|Synaptopodin antibody|SYNPO antibody|SYNPO_HUMAN antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Rat Human, Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information