Surfactant Protein A Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
surfactant protein A1	Polyclonal	IgG	Rabbit	Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Surfactant Protein A(233-248aa WNDKGCLQYRLAICEF), identical to the related rat sequence.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SFTPA1
Protein	Pulmonary surfactant-associated protein A1(PSP-A/PSPA/SP-A/SP-A1)
Uniprot ID	P35242
Function	In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.
Tissue Specificity
Sub-cellular localization	Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Sequence Similarities	Belongs to the SFTPA family.
Aliases	35 kDa pulmonary surfactant-associated protein antibody|Alveolar proteinosis protein antibody|COLEC4 antibody|Collectin 4 antibody|PSAP antibody|PSPA antibody|Pulmonary surfactant apoprotein antibody|Pulmonary surfactant-associated protein A1 antibody|SFTP1 antibody|SFTPA antibody|SFTPA1 antibody|SFTPA1B antibody|SP A antibody|SP A1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information