Plectin Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
plectin	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Plectin (2644-2671aa RFIEQEKAKLEQLFQDEVAKAQQLREEQ), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	PLEC
Protein	Plectin
Uniprot ID	Q15149
Function	Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
Tissue Specificity	Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
Sub-cellular localization	Cytoplasm, cytoskeleton.
Sequence Similarities	Belongs to the plakin or cytolinker family.
Aliases	EBS1 antibody|EBSO antibody|HD1 antibody|Hemidesmosomal protein 1 antibody|PCN antibody| pleC antibody|PLEC_HUMAN antibody|PLEC1 antibody| PLEC1b antibody|Plectin 1 antibody|plectin 1 intermediate filament binding protein 500kDa antibody|Plectin 6 antibody|Plectin antibody |Plectin-1 antibody| PLTN antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information