PF4 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
platelet factor 4	Polyclonal	IgG	Rabbit	Human	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PF4(85-101aa DLQAPLYKKIIKKLLES).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	PF4
Protein	Platelet factor 4(PF-4)
Uniprot ID	P02776
Function	Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Tissue Specificity
Sub-cellular localization	Secreted.
Sequence Similarities	Belongs to the intercrine alpha (chemokine CxC) family.
Aliases	C-X-C motif chemokine 4 antibody|Chemokine(C X C motif) ligand 4 antibody|Chemokine(CXC motif) ligand 4 antibody|CXCL 4 antibody|CXCL4 antibody|Iroplact antibody|MGC138298 antibody|Oncostatin A antibody|Oncostatin-A antibody|OncostatinA antibody|PF 4 antibody|PF-4 antibody|PF4 antibody|Platelet factor 4 antibody|PLF4_HUMAN antibody|SCYB 4 antibody|SCYB4 antibody|short form antibody|Small inducible cytokine subfamily member 4 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information