More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| glutamate receptor, ionotropic, N-methyl D-aspartate 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human NMDAR1(36-53aa RKHEQMFREAVNQANKRH), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GRIN1 |
Protein | Glutamate[NMDA] receptor subunit zeta-1 |
Uniprot ID | Q05586 |
Function | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Note: Enriched in postsynaptic plasma membrane and postsynaptic densities. |
Sequence Similarities | Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. |
Aliases | GluN1 antibody|Glutamate[NMDA] receptor subunit zeta 1 antibody|Glutamate[NMDA] receptor subunit zeta-1 antibody|Glutamate receptor ionotropic N methyl D aspartate 1 antibody|Grin 1 antibody|Grin1 antibody|MRD8 antibody|N methyl D aspartate receptor antibody|N methyl D aspartate receptor channel antibody|N methyl D aspartate receptor channel subunit zeta 1 antibody|N methyl D aspartate receptor subunit NR1 antibody|N-methyl-D-aspartate receptor antibody|N-methyl-D-aspartate receptor subunit NR1 antibody|NMD-R1 antibody|NMDA 1 antibody|NMDA NR1 antibody|NMDA R1 antibody|NMDA receptor 1 antibody|NMDA1 antibody|NMDAR 1 antibody|NMDAR antibody|Nmdar1 antibody|NMDZ1_HUMAN antibody|NR 1 antibody|NR1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NMDAR1 antibody, ASA-B1386, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Heart Tissue Lysate
Lane 5: MM453 Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: HELA Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Heart Tissue Lysate
Lane 5: MM453 Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: HELA Cell Lysate
Anti-NMDAR1 antibody, ASA-B1386, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
