NF200 Antibody (monoclonal)

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
neurofilament, heavy polypeptide	Monoclonal	IgG	Mouse	Human, Mouse, Rat	IHC-P, IHC-F, WB	Ascites
Immunogen
C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.
Concentration	1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	NEFH
Protein	Neurofilament heavy polypeptide
Uniprot ID	Human(P12036), Mouse(P19246), Rat(P16884)
Function	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Tissue Specificity
Sub-cellular localization
Sequence Similarities	Belongs to the intermediate filament family.
Aliases	NFH|KIAA0845|200 kDa neurofilament protein|Neurofilament triplet H protein

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.5ml	Human, mouse, rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	1-2μg/ml	Human, mouse, rat	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	1-2μg/ml	Human, mouse, rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information