MMP2 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
matrix metallopeptidase 2(gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, IHC-F, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MMP2(138-153aa ETVDDAFARAFQVWSD), different from the related rat and mouse sequences by two amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	MMP2
Protein	72 kDa type IV collagenase
Uniprot ID	P08253
Function	Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
Tissue Specificity	Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Sub-cellular localization	Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note: Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
Sequence Similarities	Belongs to the peptidase M10A family.
Aliases	72 kDa gelatinase antibody|72kD type IV collagenase antibody|CLG 4 antibody|CLG 4A antibody|CLG4 antibody|CLG4A antibody|Collagenase Type 4 alpha antibody|Collagenase type IV A antibody|Gelatinase A antibody|Gelatinase alpha antibody|Gelatinase neutrophil antibody|Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody|Matrix metalloproteinase 2(gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody|Matrix Metalloproteinase 2 antibody|Matrix metalloproteinase II antibody|Matrix metalloproteinase-2 antibody|MMP 2 antibody|MMP II antibody|MMP-2 antibody|MMP2 antibody|MMP2_HUMAN antibody|MONA antibody|Neutrophil gelatinase antibody|PEX antibody|TBE 1 antibody|TBE-1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Mouse, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information