MCM6 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
minichromosome maintenance complex component 6	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, IHC-F, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MCM6(589-605aa ESEDFIVEQYKHLRQRD), different from the related rat and mouse sequences by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	MCM6
Protein	DNA replication licensing factor MCM6
Uniprot ID	Q14566
Function	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Tissue Specificity
Sub-cellular localization	Nucleus. Note: Binds to chromatin during G1 and detach from it during S phase.
Sequence Similarities	Belongs to the MCM family.
Aliases	DNA replication licensing factor MCM6 antibody|MCG40308 antibody|Mcm6 antibody|MCM6 minichromosome maintenance deficient 6(MIS5 homolog, S. pombe) antibody|MCM6 minichromosome maintenance deficient 6 antibody|MCM6_HUMAN antibody|Minichromosome maintenance complex component 6 antibody|Minichromosome maintenance deficient(mis5 S. pombe) 6 antibody|Minichromosome maintenance deficient 6 homolog antibody|Minichromosome maintenance protein 6 antibody|Mis5 antibody|MIS5 homolog antibody|p105MCM antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information