MAdCAM1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
mucosal vascular addressin cell adhesion molecule 1	Polyclonal	IgG	Rabbit	Human	IHC-P, IHC-F, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MAdCAM1(173-190aa EEPQGDEDVLFRVTERWR).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	MADCAM1
Protein	Mucosal addressin cell adhesion molecule 1
Uniprot ID	Q13477
Function	Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L- selectin binding.
Tissue Specificity	Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Sub-cellular localization	Membrane; Single-pass type I membrane protein.
Sequence Similarities
Aliases	Addressin mucosal antibody|hMAdCAM 1 antibody|hMAdCAM-1 antibody|MACAM1 antibody|MADCA_HUMAN antibody|MAdCAM 1 antibody|MAdCAM-1 antibody|Madcam1 antibody|Mucosal addressin cell adhesion molecule 1 antibody|Mucosal vascular addressin cell adhesion molecule 1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information