More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| MAD1 mitotic arrest deficient-like 1 (yeast) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAD1L1 |
Protein | Mitotic spindle assembly checkpoint protein MAD1 |
Uniprot ID | Q9Y6D9 |
Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. |
Tissue Specificity | Expressed weakly at G0/G1 and highly at late S and G2/M phase. |
Sub-cellular localization | Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note: From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore. |
Sequence Similarities | Belongs to the MAD1 family. |
Aliases | hMAD1 antibody|HsMAD1 antibody|MAD1 antibody|MAD1 mitotic arrest deficient like 1 (yeast) antibody|MAD1-like protein 1 antibody|MAD1L1 antibody|MD1L1_HUMAN antibody|Mitotic arrest deficient 1 antibody|Mitotic arrest deficient 1-like protein 1 antibody|Mitotic checkpoint MAD1 protein homolog antibody|Mitotic spindle assembly checkpoint protein MAD1 antibody|PIG9 antibody|Tax binding protein 181 antibody|Tax-binding protein 181 antibody|TP53I9 antibody|Tumor protein p53 inducible protein 9 antibody|TXBP181 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MAD1 antibody, ASA-B1204, Western blotting
All lanes: Anti MAD1 (ASA-B1204) at 0.5ug/ml
WB: Recombinant Human MAD1 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
All lanes: Anti MAD1 (ASA-B1204) at 0.5ug/ml
WB: Recombinant Human MAD1 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Anti- MAD1 antibody, ASA-B1204, Western blotting
All lanes: Anti MAD1 (ASA-B1204) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: SHG Whole Cell Lysate at 40ug
Lane 6: 22RV1 Whole Cell Lysate at 40ug
Lane 7: PANC Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
All lanes: Anti MAD1 (ASA-B1204) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: SHG Whole Cell Lysate at 40ug
Lane 6: 22RV1 Whole Cell Lysate at 40ug
Lane 7: PANC Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
Anti- MAD1 antibody, ASA-B1204, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue