Kallistatin Antibody View larger

Kallistatin Antibody

ASA-B1100

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4 Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Kallistatin(384-401aa AIKFFSAQTNRHILRFNR).

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SERPINA4

Protein

Kallistatin

Uniprot ID

P29622

Function

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

Tissue Specificity

Expressed by the liver and secreted in plasma.

Sub-cellular localization

Secreted.

Sequence Similarities

Belongs to the serpin family.

Aliases

KAIN_HUMAN antibody|KAL antibody|Kallikrein binding protein antibody|Kallikrein inhibitor antibody|Kallistatin antibody|KBP antibody|KLST antibody|KST antibody|MGC108582 antibody|Peptidase inhibitor 4 antibody|PI-4 antibody|PI4 antibody|Protease inhibitor 4(kallistatin) antibody|Protease inhibitor 4 antibody|Serine(or cysteine) proteinase inhibitor, clade A(alpha 1 antiproteinase, antitrypsin), member 4 antibody|Serpin A4 antibody|Serpin peptidase inhibitor clade A member 4 antibody|Serpin peptidase inhibitor, clade A(alpha 1 antiproteinase, antitrypsin), member 4 antibody|SERPINA4 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Kallistatin antibody, ASA-B1100, IHC(P)
IHC(P): Human Liver Cancer Tissue
Anti-Kallistatin antibody, ASA-B1100, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: JURKAT Cell Lysate
Anti-Kallistatin antibody, ASA-B1100, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SKOV Cell Lysate

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