More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| kallikrein-related peptidase 11 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 11(233-250aa YTKVCKYVDWIQETMKNN). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KLK11 |
Protein | Kallikrein-11(hK11) |
Uniprot ID | Q9UBX7 |
Function | Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. |
Tissue Specificity | Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). |
Sub-cellular localization | Isoform 1: Secreted. |
Sequence Similarities | Belongs to the peptidase S1 family. Kallikrein subfamily. |
Aliases | Hippostasin antibody|hK11 antibody|Kallikrein 11 antibody|Kallikrein 11 isoform 1 preproprotein antibody|Kallikrein 11 isoform 2 precursor antibody|Kallikrein 11 precursor antibody|Kallikrein related peptidase 11 antibody|Kallikrein-11 inactive chain 2 antibody|KLK 11 antibody|Klk11 antibody|KLK11_HUMAN antibody|MGC33060 antibody|Protease serine 20 trypsin like antibody|Protease serine trypsin like antibody|PRSS20 antibody|Serine protease 20 antibody|TLSP antibody|Trypsin like protease antibody|Trypsin-like protease antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Kallikrein 11 antibody, ASA-B1094, Western blotting
All lanes: Anti Kallikrein 11 (ASA-B1094) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 31KD
All lanes: Anti Kallikrein 11 (ASA-B1094) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 31KD
Anti-Kallikrein 11 antibody, ASA-B1094, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
