More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| intercellular adhesion molecule 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human ICAM1 recombinant protein (Position: L214-P532). Human ICAM1 shares 52% and 48% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ICAM1 |
Protein | Intercellular adhesion molecule 1 |
Uniprot ID | P05362 |
Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the immunoglobulin superfamily. ICAM family. |
Aliases | Antigen identified by monoclonal antibody BB2 antibody|BB 2 antibody|BB2 antibody|CD 54 antibody|CD_antigen=CD54 antibody|CD54 antibody|CD54 antigen antibody|Cell surface glycoprotein P3.58 antibody|Human rhinovirus receptor antibody|ICAM 1 antibody|ICAM-1 antibody|ICAM1 antibody|ICAM1_HUMAN antibody|intercellular adhesion molecule 1(CD54)|human rhinovirus receptor antibody|Intercellular adhesion molecule 1 antibody|Major group rhinovirus receptor antibody|MALA 2 antibody|MALA2 antibody|MyD 10 antibody|MyD10 antibody|P3.58 antibody|Surface antigen of activated B cells antibody|Surface antigen of activated B cells, BB2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ICAM1 antibody, ASA-B0964--1.jpg
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-ICAM1 antibody, ASA-B0964--2.jpg
All lanes: Anti-ICAM1(ASA-B0964) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: K562 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 100KD
All lanes: Anti-ICAM1(ASA-B0964) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: K562 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 100KD
