Hsp47 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)	Polyclonal	IgG	Rabbit	Human, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SERPINH1
Protein	Serpin H1
Uniprot ID	P50454
Function	Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Tissue Specificity
Sub-cellular localization	Endoplasmic reticulum lumen.
Sequence Similarities	Belongs to the serpin family.
Aliases	47 kDa heat shock protein antibody|47 kDa heat shock protein precursor antibody|Arsenic transactivated protein 3 antibody|Arsenic-transactivated protein 3 antibody|AsTP 3 antibody|AsTP3 antibody|CBP 1 antibody|CBP 2 antibody|CBP1 antibody|CBP2 antibody|Cell proliferation-inducing gene 14 protein antibody|Collagen binding protein 1 antibody|Collagen binding protein 2 antibody|Collagen binding protein antibody|Collagen-binding protein 2 antibody|Collagen-binding protein antibody|Colligen antibody|Colligin 1 antibody|Colligin 2 antibody|Colligin antibody|colligin-1 antibody|colligin-2 antibody|gp46 antibody|Heat shock protein 47 antibody|Heat-shock protein 47 antibody|HGNC 1547 antibody|Hsp 47 antibody|J6 antibody|OI10 antibody|PIG 14 antibody|PIG14 antibody|PPROM antibody|Proliferation inducing gene 14 antibody|Proliferation inducing gene 14 protein antibody|RA A47 antibody|RA-A47 antibody|Rheumatoid arthritis antigen A 47 antibody|rheumatoid arthritis antigen A-47 antibody|Rheumatoid arthritis related antigen RA A47 antibody|Rheumatoid arthritis-related antigen RA-A47 antibody|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody|Serine or cysteine proteinase inhibitor clade H member 1 antibody|Serine or cysteine proteinase inhibitor clade H member 2 antibody|SERPH_HUMAN antibody|Serpin H1 antibody|Serpin peptidase inhibitor clade H member 1 antibody|serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody|Serpin peptidase inhibitor, clade H, member 1 antibody|SERPINH1 antibody|SERPINH2 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information